There Are Two Main Forms Of Disposable Virus Sampling Tube Preservation Solution

There are two main forms of disposable virus sampling tube preservation solutions. There are two main types of preservation solutions for virus blood collection tubes widely used in the market, one is a virus maintenance solution improved based on the transport medium, and the other is a preservation solution improved by nucleic acid extraction and lysate.

The main component of the former is Eagle's basal medium (MEM) or Hank's balanced salt, adding salts, amino acids, vitamins, glucose, and protein required for virus survival. This preservation solution uses phenol red sodium salt as an indicator, and the solution is pink when the pH value is 6.6-8.0. Necessary glucose, L-glutamine, and protein are added to the preservation solution. The protein is provided in the form of a fetal bovine serum or bovine serum albumin, which can stabilize the protein coat of the virus. Because the preservation solution is rich in nutrients, it is conducive to the survival of viruses but also to the growth of bacteria. If bacteria are contaminated in the preservation solution, they will multiply in large numbers, and the carbon dioxide in their metabolites will reduce the pH value of the preservation solution, from pink to pink. turns yellow. Therefore, most manufacturers have added antibacterial ingredients to the formula. The recommended antibacterial agents are penicillin, streptomycin, gentamicin, and polymyxin B. Sodium azide, and 2-formal are not recommended. Inhibitors such as methyl-4-isothiazolinone-3-one (MCI) and 5-chloro-2-methyl-4-isothiazolin-3-one (CMCI), because these components have an impact on the PCR reaction. Since the sample provided by this preservation solution is basically a live virus, the originality of the sample can be kept to the greatest extent, and it can be used not only for nucleic acid extraction and detection of the virus but also for the cultivation and isolation of the virus. However, when used for detection, it should be noted that nucleic acid extraction and purification must be performed after inactivation.

Another kind of preservation solution based on nucleic acid extraction lysate, the main components are balanced salts, EDTA chelating agent, guanidine salt (such as guanidine isothiocyanate, guanidine hydrochloride, etc.), anionic surfactants (such as dodecane Sodium sulfate), cationic surfactant (such as tetradecyltrimethylammonium oxalate), phenol, 8-hydroxyquinoline, dithiothreitol (DTT), proteinase K and other several or more components, This preservation solution directly lyses the virus to release nucleic acid and eliminates nucleolytic enzyme (RNase). If it is only used for RT-PCR, it is more suitable, but the lysate can inactivate the virus, and this kind of sample cannot be used for virus culture and isolation.

It is recommended to use EDTA salts (such as dipotassium ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetic acid, etc.) as the metal ion chelating agent used in the preservation solution of disposable protective virus blood collection tubes. It is not recommended to use heparins (such as sodium heparin, lithium heparin, etc.) ), so as not to affect the PCR detection.